Radioimmunoassay of human growth hormone: technique and application to plasma, cerebrospinal fluid, and pituitary extracts

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[Radioimmunoassay for human growth hormone].

Detailedmethodsfor: (1) 1311 iodination of human growth hormone (HGH); (2) purification of labeled HGH (HGH-139); and (3) radioimmunoassay of HGH are given. Bio-GeI filtration is introduced as a rapid and reproducible method for purifying HGH-1311which hasbeenobtainedby a modificationof the methodof Greenwood at al. (1). Highlypurified HGH-’311 with a bindability of 96% or more is usually achie...

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State of human growth hormone in plasma and changes in stored solutions of pituitary growth hormone.

Radioimmunoassay of endogenous plasma growth hormone in human plasma fractions separated by starch gel electrophoresis shows that the plasma hormone migrates in a fairly narrow peak behind serum albumin. A similar electrophoretic mobility is exhibited by certain pituitary growth hormone preparations (the A type of Wilhehni), added to plasma in physiological concentrations or on electrophoresis ...

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[Radioimmunoassay of human growth hormone by adsorption to talc].

Radioimmunoassay of human growth hormone (HGH) using adsorption to talc was examined. Results are as follows: 1) The minimal detectable amount of HGH was 0.02 ng/ml. The mean recovery rate was 100.8%. Mean +standard error (S.E.) of the same two samples, repeatedly measured eight times, were 3.5+0.2 and 35.4+0.5 ng/ml, respectively . Concentrations of the same acromegalic plasma diluted in diffe...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1972

ISSN: 0021-9746

DOI: 10.1136/jcp.25.9.774